Multiple regulation of carp (Cyprinus carpio L.) macrophages and neutrophilic granulocytes by serum factors: influence of infection with atypical Aeromonas salmonicida.
نویسندگان
چکیده
Normal carp serum contains inhibitory and stimulatory factors for macrophage and neutrophilic granulocyte respiratory burst activity. As stimulatory factors were only effective in combination with phorbol myristate actetate (PMA) activation, it is concluded that they are probably linked to protein kinase C activation. Both the stimulatory and inhibitory factors are heat stable. Macrophage- and neutrophilic granulocyte-enriched cell fractions from the pronephros of carp had high respiratory burst- and high bactericidal in vitro responses to virulent atypical Aeromonas salmonicida bacteria. Serum factors were inhibitory for the A. salmonicida induced respiratory burst activity. No change in inhibitory or stimulatory serum factors could be observed during a 12-day challenge experiment with A. salmonicida, or during a rechallenge of survivors from a previous sub-lethal infection. The sensitivity of macrophages and neutrophilic granulocytes to stimulation of respiratory burst activity by PMA was not significantly altered. Culture supernatants from PHA pre-treated lymphocytes stimulated the respiratory burst activity of macrophages and neutrophilic granulocytes suggesting that serum factors may partially be lymphocyte derived.
منابع مشابه
Protection against Atypical Aeromonas salmonicida Infection in Carp (Cyprinus carpio L.) by Oral Administration of Probiotic Bacterial Culture
Introduction Atypical Aeromonas salmonicida is a fish pathogen that causes several diseases in freshwater fish such as goldfish (Carassius auratus L.) (6) and eel (Anguilla japonica) (2), and also seawater fish (5). Since 1996, A. salmonicida infection characterized by the formation of ulcers on the body surface and fins (ulcer disease) has become prevalent in colored carp (Cyprinus carpio koi ...
متن کاملOccurrence and significance of atypical Aeromonas salmonicida in non-salmonid and salmonid fish species: a review.
Bacterial strains of Aeromonas salmonicida included in the recognized subsp. acromogenes, subsp. masoucida, and subsp. smithia in addition to the large number of strains not included in any of the described subspecies are referred to as atypical A. salmonicida. The atypical strains form a very heterogeneous group with respect to biochemical characteristics, growth conditions, and production of ...
متن کاملPhenotypic characteristics and pathogenicity of Aeromonas genomospecies isolated from common carp (Cyprinus carpio L.).
AIMS To evaluate the relationship between the genomospecies, phenotypic profile and pathogenicity for carp of 37 motile Aeromonas strains. METHODS AND RESULTS Aeromonas strains were identified to genomospecies level by the 16S rDNA restriction fragment length polymorphism (RFLP) method and characterized phenotypically by the API 20E and API Zym systems and by conventional tube or plate method...
متن کاملCharacterization of Macrophages and Neutrophilic Granulocytes from the Pronephros of Carp (cyprinus Carpio)
To analyse the functional activity of different leucocyte types, carp pronephros cells were separated on Percoll density gradients and by use of fluorescence-activated cell sorting. Cell populations were characterised by light and electron microscopy and by flow cytometry. Fractions enriched in macrophages and neutrophilic granulocytes were subsequently analysed for phagocytic activity in vitro...
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عنوان ژورنال:
- Veterinary immunology and immunopathology
دوره 51 1-2 شماره
صفحات -
تاریخ انتشار 1996